v5 rabbit polyclonal 13202 antibody Search Results


secondary antibody conjugated to ir dye 800cw  (LI-COR)
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LI-COR secondary antibody conjugated to ir dye 800cw
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rabbit anti v5  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc rabbit anti v5
Rabbit Anti V5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit mab  (Addgene inc)
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Addgene inc rabbit mab
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v5 13202 dnmt1 5032  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc v5 13202 dnmt1 5032
V5 13202 Dnmt1 5032, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti v5 tag  (New England Biolabs)
86
New England Biolabs rabbit anti v5 tag
(A to C) Expression <t>of</t> <t>Hspb8/HSPB8</t> in WT MEFs (A), Atf4–/– MEFs (B), and scrambled, HRI KD, and GCN2 KD HeLa cells (C) infected with Shigella or treated with 5 μM thapsigargin for 4 hours. (D) Expression of IL8 in scrambled or HSPB8 KD (shHSPB8) HeLa cells infected with Shigella. (E) Expression of IL8 in scrambled or HRI KD HeLa cells infected 4 hours with Shigella with or without transfection of <t>HSPB8-V5,</t> as measured by qPCR. (F) Coimmunoprecipitation experiments with unstimulated (CTR) or Shigella-infected HeLa cell lysates. (G) Coimmunoprecipitation experiments with the lysates of HeLa cells that were transfected with NOD1-HA and HSPB8-V5 and infected with Shigella. (H) Scrambled or HRI KD HEK293T cells transfected with NOD1-HA with or without HSPB8-V5 and analyzed as in Fig. 3A. (I and J) Scrambled or HRI KD HEK293T cells transfected with or without HSPB8-V5 (I) and scrambled or HSPB8 KD HEK293T cells (J) were stimulated for 30 min with C12-iE-DAP (10 ng/ml) and NOD1-HA complexes were subjected to SYPRO Orange hydrophobicity assays. Graphs display means ± SD from three to five independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Asterisk in (F) denotes a nonspecific band.
Rabbit Anti V5 Tag, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti v5  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti v5
(A to C) Expression <t>of</t> <t>Hspb8/HSPB8</t> in WT MEFs (A), Atf4–/– MEFs (B), and scrambled, HRI KD, and GCN2 KD HeLa cells (C) infected with Shigella or treated with 5 μM thapsigargin for 4 hours. (D) Expression of IL8 in scrambled or HSPB8 KD (shHSPB8) HeLa cells infected with Shigella. (E) Expression of IL8 in scrambled or HRI KD HeLa cells infected 4 hours with Shigella with or without transfection of <t>HSPB8-V5,</t> as measured by qPCR. (F) Coimmunoprecipitation experiments with unstimulated (CTR) or Shigella-infected HeLa cell lysates. (G) Coimmunoprecipitation experiments with the lysates of HeLa cells that were transfected with NOD1-HA and HSPB8-V5 and infected with Shigella. (H) Scrambled or HRI KD HEK293T cells transfected with NOD1-HA with or without HSPB8-V5 and analyzed as in Fig. 3A. (I and J) Scrambled or HRI KD HEK293T cells transfected with or without HSPB8-V5 (I) and scrambled or HSPB8 KD HEK293T cells (J) were stimulated for 30 min with C12-iE-DAP (10 ng/ml) and NOD1-HA complexes were subjected to SYPRO Orange hydrophobicity assays. Graphs display means ± SD from three to five independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Asterisk in (F) denotes a nonspecific band.
Anti V5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d3h8q cell signaling ha epitope  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc d3h8q cell signaling ha epitope
(A to C) Expression <t>of</t> <t>Hspb8/HSPB8</t> in WT MEFs (A), Atf4–/– MEFs (B), and scrambled, HRI KD, and GCN2 KD HeLa cells (C) infected with Shigella or treated with 5 μM thapsigargin for 4 hours. (D) Expression of IL8 in scrambled or HSPB8 KD (shHSPB8) HeLa cells infected with Shigella. (E) Expression of IL8 in scrambled or HRI KD HeLa cells infected 4 hours with Shigella with or without transfection of <t>HSPB8-V5,</t> as measured by qPCR. (F) Coimmunoprecipitation experiments with unstimulated (CTR) or Shigella-infected HeLa cell lysates. (G) Coimmunoprecipitation experiments with the lysates of HeLa cells that were transfected with NOD1-HA and HSPB8-V5 and infected with Shigella. (H) Scrambled or HRI KD HEK293T cells transfected with NOD1-HA with or without HSPB8-V5 and analyzed as in Fig. 3A. (I and J) Scrambled or HRI KD HEK293T cells transfected with or without HSPB8-V5 (I) and scrambled or HSPB8 KD HEK293T cells (J) were stimulated for 30 min with C12-iE-DAP (10 ng/ml) and NOD1-HA complexes were subjected to SYPRO Orange hydrophobicity assays. Graphs display means ± SD from three to five independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Asterisk in (F) denotes a nonspecific band.
D3h8q Cell Signaling Ha Epitope, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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irs 1 59g8 rabbit mab immunoblot analysis cell signaling technology  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc irs 1 59g8 rabbit mab immunoblot analysis cell signaling technology
(A to C) Expression <t>of</t> <t>Hspb8/HSPB8</t> in WT MEFs (A), Atf4–/– MEFs (B), and scrambled, HRI KD, and GCN2 KD HeLa cells (C) infected with Shigella or treated with 5 μM thapsigargin for 4 hours. (D) Expression of IL8 in scrambled or HSPB8 KD (shHSPB8) HeLa cells infected with Shigella. (E) Expression of IL8 in scrambled or HRI KD HeLa cells infected 4 hours with Shigella with or without transfection of <t>HSPB8-V5,</t> as measured by qPCR. (F) Coimmunoprecipitation experiments with unstimulated (CTR) or Shigella-infected HeLa cell lysates. (G) Coimmunoprecipitation experiments with the lysates of HeLa cells that were transfected with NOD1-HA and HSPB8-V5 and infected with Shigella. (H) Scrambled or HRI KD HEK293T cells transfected with NOD1-HA with or without HSPB8-V5 and analyzed as in Fig. 3A. (I and J) Scrambled or HRI KD HEK293T cells transfected with or without HSPB8-V5 (I) and scrambled or HSPB8 KD HEK293T cells (J) were stimulated for 30 min with C12-iE-DAP (10 ng/ml) and NOD1-HA complexes were subjected to SYPRO Orange hydrophobicity assays. Graphs display means ± SD from three to five independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Asterisk in (F) denotes a nonspecific band.
Irs 1 59g8 Rabbit Mab Immunoblot Analysis Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-v5 antibody #13202  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc rabbit anti-v5 antibody #13202
(A to C) Expression <t>of</t> <t>Hspb8/HSPB8</t> in WT MEFs (A), Atf4–/– MEFs (B), and scrambled, HRI KD, and GCN2 KD HeLa cells (C) infected with Shigella or treated with 5 μM thapsigargin for 4 hours. (D) Expression of IL8 in scrambled or HSPB8 KD (shHSPB8) HeLa cells infected with Shigella. (E) Expression of IL8 in scrambled or HRI KD HeLa cells infected 4 hours with Shigella with or without transfection of <t>HSPB8-V5,</t> as measured by qPCR. (F) Coimmunoprecipitation experiments with unstimulated (CTR) or Shigella-infected HeLa cell lysates. (G) Coimmunoprecipitation experiments with the lysates of HeLa cells that were transfected with NOD1-HA and HSPB8-V5 and infected with Shigella. (H) Scrambled or HRI KD HEK293T cells transfected with NOD1-HA with or without HSPB8-V5 and analyzed as in Fig. 3A. (I and J) Scrambled or HRI KD HEK293T cells transfected with or without HSPB8-V5 (I) and scrambled or HSPB8 KD HEK293T cells (J) were stimulated for 30 min with C12-iE-DAP (10 ng/ml) and NOD1-HA complexes were subjected to SYPRO Orange hydrophobicity assays. Graphs display means ± SD from three to five independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Asterisk in (F) denotes a nonspecific band.
Rabbit Anti V5 Antibody #13202, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody rabbit anti p44 42 mapk erk1 2 cell signaling technology  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc antibody rabbit anti p44 42 mapk erk1 2 cell signaling technology
(A to C) Expression <t>of</t> <t>Hspb8/HSPB8</t> in WT MEFs (A), Atf4–/– MEFs (B), and scrambled, HRI KD, and GCN2 KD HeLa cells (C) infected with Shigella or treated with 5 μM thapsigargin for 4 hours. (D) Expression of IL8 in scrambled or HSPB8 KD (shHSPB8) HeLa cells infected with Shigella. (E) Expression of IL8 in scrambled or HRI KD HeLa cells infected 4 hours with Shigella with or without transfection of <t>HSPB8-V5,</t> as measured by qPCR. (F) Coimmunoprecipitation experiments with unstimulated (CTR) or Shigella-infected HeLa cell lysates. (G) Coimmunoprecipitation experiments with the lysates of HeLa cells that were transfected with NOD1-HA and HSPB8-V5 and infected with Shigella. (H) Scrambled or HRI KD HEK293T cells transfected with NOD1-HA with or without HSPB8-V5 and analyzed as in Fig. 3A. (I and J) Scrambled or HRI KD HEK293T cells transfected with or without HSPB8-V5 (I) and scrambled or HSPB8 KD HEK293T cells (J) were stimulated for 30 min with C12-iE-DAP (10 ng/ml) and NOD1-HA complexes were subjected to SYPRO Orange hydrophobicity assays. Graphs display means ± SD from three to five independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Asterisk in (F) denotes a nonspecific band.
Antibody Rabbit Anti P44 42 Mapk Erk1 2 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti-tlr8 antibody # ddx0480p clone 303f1.14  (Novus Biologicals)
90
Novus Biologicals mouse anti-tlr8 antibody # ddx0480p clone 303f1.14
(A to C) Expression <t>of</t> <t>Hspb8/HSPB8</t> in WT MEFs (A), Atf4–/– MEFs (B), and scrambled, HRI KD, and GCN2 KD HeLa cells (C) infected with Shigella or treated with 5 μM thapsigargin for 4 hours. (D) Expression of IL8 in scrambled or HSPB8 KD (shHSPB8) HeLa cells infected with Shigella. (E) Expression of IL8 in scrambled or HRI KD HeLa cells infected 4 hours with Shigella with or without transfection of <t>HSPB8-V5,</t> as measured by qPCR. (F) Coimmunoprecipitation experiments with unstimulated (CTR) or Shigella-infected HeLa cell lysates. (G) Coimmunoprecipitation experiments with the lysates of HeLa cells that were transfected with NOD1-HA and HSPB8-V5 and infected with Shigella. (H) Scrambled or HRI KD HEK293T cells transfected with NOD1-HA with or without HSPB8-V5 and analyzed as in Fig. 3A. (I and J) Scrambled or HRI KD HEK293T cells transfected with or without HSPB8-V5 (I) and scrambled or HSPB8 KD HEK293T cells (J) were stimulated for 30 min with C12-iE-DAP (10 ng/ml) and NOD1-HA complexes were subjected to SYPRO Orange hydrophobicity assays. Graphs display means ± SD from three to five independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Asterisk in (F) denotes a nonspecific band.
Mouse Anti Tlr8 Antibody # Ddx0480p Clone 303f1.14, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-v5 (rabbit) 13202  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc anti-v5 (rabbit) 13202
(A to C) Expression <t>of</t> <t>Hspb8/HSPB8</t> in WT MEFs (A), Atf4–/– MEFs (B), and scrambled, HRI KD, and GCN2 KD HeLa cells (C) infected with Shigella or treated with 5 μM thapsigargin for 4 hours. (D) Expression of IL8 in scrambled or HSPB8 KD (shHSPB8) HeLa cells infected with Shigella. (E) Expression of IL8 in scrambled or HRI KD HeLa cells infected 4 hours with Shigella with or without transfection of <t>HSPB8-V5,</t> as measured by qPCR. (F) Coimmunoprecipitation experiments with unstimulated (CTR) or Shigella-infected HeLa cell lysates. (G) Coimmunoprecipitation experiments with the lysates of HeLa cells that were transfected with NOD1-HA and HSPB8-V5 and infected with Shigella. (H) Scrambled or HRI KD HEK293T cells transfected with NOD1-HA with or without HSPB8-V5 and analyzed as in Fig. 3A. (I and J) Scrambled or HRI KD HEK293T cells transfected with or without HSPB8-V5 (I) and scrambled or HSPB8 KD HEK293T cells (J) were stimulated for 30 min with C12-iE-DAP (10 ng/ml) and NOD1-HA complexes were subjected to SYPRO Orange hydrophobicity assays. Graphs display means ± SD from three to five independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Asterisk in (F) denotes a nonspecific band.
Anti V5 (Rabbit) 13202, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A to C) Expression of Hspb8/HSPB8 in WT MEFs (A), Atf4–/– MEFs (B), and scrambled, HRI KD, and GCN2 KD HeLa cells (C) infected with Shigella or treated with 5 μM thapsigargin for 4 hours. (D) Expression of IL8 in scrambled or HSPB8 KD (shHSPB8) HeLa cells infected with Shigella. (E) Expression of IL8 in scrambled or HRI KD HeLa cells infected 4 hours with Shigella with or without transfection of HSPB8-V5, as measured by qPCR. (F) Coimmunoprecipitation experiments with unstimulated (CTR) or Shigella-infected HeLa cell lysates. (G) Coimmunoprecipitation experiments with the lysates of HeLa cells that were transfected with NOD1-HA and HSPB8-V5 and infected with Shigella. (H) Scrambled or HRI KD HEK293T cells transfected with NOD1-HA with or without HSPB8-V5 and analyzed as in Fig. 3A. (I and J) Scrambled or HRI KD HEK293T cells transfected with or without HSPB8-V5 (I) and scrambled or HSPB8 KD HEK293T cells (J) were stimulated for 30 min with C12-iE-DAP (10 ng/ml) and NOD1-HA complexes were subjected to SYPRO Orange hydrophobicity assays. Graphs display means ± SD from three to five independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Asterisk in (F) denotes a nonspecific band.

Journal: Science (New York, N.Y.)

Article Title: The heme-regulated inhibitor is a cytosolic sensor of protein misfolding that controls innate immune signaling

doi: 10.1126/science.aaw4144

Figure Lengend Snippet: (A to C) Expression of Hspb8/HSPB8 in WT MEFs (A), Atf4–/– MEFs (B), and scrambled, HRI KD, and GCN2 KD HeLa cells (C) infected with Shigella or treated with 5 μM thapsigargin for 4 hours. (D) Expression of IL8 in scrambled or HSPB8 KD (shHSPB8) HeLa cells infected with Shigella. (E) Expression of IL8 in scrambled or HRI KD HeLa cells infected 4 hours with Shigella with or without transfection of HSPB8-V5, as measured by qPCR. (F) Coimmunoprecipitation experiments with unstimulated (CTR) or Shigella-infected HeLa cell lysates. (G) Coimmunoprecipitation experiments with the lysates of HeLa cells that were transfected with NOD1-HA and HSPB8-V5 and infected with Shigella. (H) Scrambled or HRI KD HEK293T cells transfected with NOD1-HA with or without HSPB8-V5 and analyzed as in Fig. 3A. (I and J) Scrambled or HRI KD HEK293T cells transfected with or without HSPB8-V5 (I) and scrambled or HSPB8 KD HEK293T cells (J) were stimulated for 30 min with C12-iE-DAP (10 ng/ml) and NOD1-HA complexes were subjected to SYPRO Orange hydrophobicity assays. Graphs display means ± SD from three to five independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Asterisk in (F) denotes a nonspecific band.

Article Snippet: Membranes were blocked with 5% milk in TBST and incubated with the indicated antibodies: mouse anti-tubulin (#T5168, Sigma, 1/10,000 dilution), rabbit anti-eIF2α (#9722S, NEB, 1/1000), rabbit anti-phospho-eIF2α (#9721, NEB, 1/1000), rabbit anti-HRI (#07–226, Upstate, 1/5000), anti-HRI polyclonal ( 6 ) (1/3000) rabbit anti-GCN2 (#ab137543, Abcam, 1/1000), monoclonal anti-HA tag (#G036, Abcam, 1/1000), monoclonal anti-Flag-Tag (#F3165, Sigma, 1/1000), rabbit anti-V5-Tag (#13202, NEB, 1/1000), rabbit anti-HSPB8 (#3059, cell signaling, 1/500), rabbit anti-HSP90 (#ab13492, Abcam, 1/3000), rabbit anti-Hsc70 (#ab51052, Abcam, 1/1000), rabbit anti-ERK (#9102, NEB, 1/4000), mouse anti-phospho-ERK (#9106, NEB, 1/4000), mouse anti-IkBα (#4814, NEB, 1/4000), rabbit anti-IkBα (# 9242, NEB, 1/2000), mouse anti-phospho-IkBα (#9246, NEB, 1/2000),rabbit anti-GAPDH (#G9545,Sigma,1/20,000), mouseanti-caspase-1(p20) (#AG-20B-0042, Adipogen, 1/4000), mouse anti-NLRP3 (#AG-20B-0014, Adipogen, 1/4000), goat anti-mouse IL-1β (#AF-401-NA, R&D Systems, 1/1000), rabbit anti-IRF3 (#4302, NEB, 1/2000), rabbit anti-phospho-IRF3 (Ser396) (#4947, NEB), rabbit anti-NAK/TBK1 (EP611Y) (#ab40676, Abcam, 1/4000), rabbit anti-phospho-TBK1 (Ser172) (EPR2867(2)) (#ab109272, Abcam, 1/2000) or rabbit anti-phospho-TBK1/NAK (Ser172) (#5483, NEB, 1/2000), rabbit anti-MAVS (#4983, NEB, 1/2000) and rabbit anti-MAVS (M-300) (#sc-68881, Santa Cruz, 1/1000), rabbit anti-STING/TMEM173 (#19851–1-AP, Proteintech, 1/2000), rabbit anti-ASC mouse specific (D2W8U) (#67824, NEB, 1/2000), rabbit polyclonal anti-ATF3 (H-90) (#sc22978, Santa Cruz, 1/1000), rabbit anti-ATF4 (D4B8) (#11815, NEB, 1/1000), mouse monoclonal anti-puromycin (4G11) (#MABE342, Millipore, 1/5000), rabbit anti-α-synuclein phosphoS129 (#ab51253, Abcam, 1/3000), mouse anti-α-synuclein (#ab1903, Abcam, 1/3000).

Techniques: Expressing, Infection, Transfection